Publications

2021

  • Hirschmann S,Gómez-Mejia A, Mäder U, Karsunke J, Driesch D, Rohde M, Häussler S, Burchhardt G, Hammerschmidt S.  The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression. Microorganisms. 2021 Feb 24;9(3):468. doi: 10.3390/microorganisms9030468. PMID: 33668344

2020

  • Abdurrahman G, Schmiedeke F, Bachert C, Bröker B, Holtfreter S (2020) Allergy-A New Role for T Cell Superantigens of Staphylococcus aureus? Toxins. 2020; 12(3).

  • Hirschfeld C, Gómez-Mejia A, Bartel J, Hentschker C, Rohde M, Maaß S, Hammerschmidt S, Becher D. Proteomic Investigation Uncovers Potential Targets and Target Sites of Pneumococcal Serine-Threonine Kinase StkP and Phosphatase PhpP. Front Microbiol 2020 Feb 4;10:3101. doi: 10.3389/fmicb.2019.03101. eCollection 2019. PubMed

  • Mücke PA, Maaß S, Kohler TP, Hammerschmidt S, Becher D. Proteomic Adaptation of Streptococcus pneumoniaeto the Human Antimicrobial Peptide LL-37. Microorganisms 8(3). pii: E413. doi: 10.3390/microorganisms8030413. PubMed

  • SchultzD, Surabhi S, Stelling N, Rothe M, KoInfekt Study Group, Methling K, HammerschmidtS, Siemens N, Lalk M. 16HBE Cell Lipid Mediator Responses to Mono and Co-Infections with Respiratory Pathogens. Metabolites 2020, 10(3), 113; doi.org/10.3390/metabo10030113. PubMed

  • Leonard A, Möhlis K, Schlüter R, Taylor E, Lalk M, Methling K. Exploring metabolic adaptation of Streptococcus pneumoniae to antibiotics. The Journal of Antibiotics volume 73, pages441–454(2020). PubMed
  • Voß F, van Beek LF, Schwudke D, Ederveen THA, van Opzeeland FJ, Thalheim D, Werner S, de Jonge MI, Hammerschmidt S. Lipidation of Pneumococcal Antigens Leads to Improved Immunogenicity and Protection. Vaccines (Basel) 2020 Jun 17;8(2):E310. doi: 10.3390/vaccines8020310. PubMed

2019

  • Palma Medina LM, Becker A, Michalik S, Yedavally H, Raineri EJM, Hildebrandt P, Gesell Salazar M, Surmann K, Pförtner H, Mekonnen SA, Salvati A, Kaderali L, van Dijl JM, Völker U (2019) Metabolic Cross-talk Between Human Bronchial Epithelial Cells and Internalized Staphylococcus aureus as a Driver for Infection. Mol Cell Proteomics. 18:892-908. MCP
  • Schultz D, Methling K; KoInfekt Study Group, Rothe M, Lalk M.Eicosanoid Profile of Influenza A Virus Infected Pigs. Metabolites. 2019 Jul 3;9(7). pii: E130. doi: 10.3390/metabo9070130. PubMed
  • Seinen J, Dieperink W, Mekonnen SA, Lisotto P, Harmsen HJM, Hiemstra B, Ott A, Schultz D, Lalk M, Oswald S, Hammerschmidt S*,de Smet AMGA, van Dijl JM*. (2019) Heterogeneous antimicrobial activity in broncho-alveolar aspirates from mechanically ventilated intensive care unit patients. Virulence 10:879-891. PubMed

2018

  • Nagel A, Michalik S, Debarbouille M, Hertlein T, Gesell Salazar M, Rath H, Msadek T, Ohlsen K, van Dijl JM, Völker U, Mäder U (2018) Inhibition of Rho Activity Increases Expression of SaeRS-Dependent Virulence Factor Genes in Staphylococcus aureus, Showing a Link between Transcription Termination, Antibiotic Action, and Virulence. MBio. 9:1-17.
  • Jarick M, Bertsche U, Stahl M, Schultz D, Methling K, Lalk M, Stigloher C, Steger M, Schlosser A, Ohlsen K. The serine/threonine kinase Stk and the phosphatase Stp regulate cell wall synthesis in Staphylococcus aureus. Sci Rep 2018; 8(1):13693. doi: 10.1038/s41598-018-32109-7. PubMed
  • Leonard A, Lalk M. Infection and metabolism - Streptococcus pneumoniae metabolism facing the host environment. Cytokine 2018; pii: S1043-4666(18)30319-3. doi: 10.1016/j.cyto.2018.07.021. [Epub ahead of print] PubMed
  • Gómez-Mejia A, Gámez G, Hirschmann S, Kluger V, Rath H, Böhm S, Voss F, Kakar N, Petruschka L, Völker U, Brückner R, Mäder U, Hammerschmidt S. Pneumococcal Metabolic Adaptation and Colonization Are Regulated by the Two-Component Regulatory System 08. mSphere 2018; 3(3). pii: e00165-18. doi: 10.1128/mSphere.00165-18. PubMed
  • Nordengrün M, Michalik S, Völker U,  Bröker BM, Gómez-Gascón L. The quest for bacterial allergens. Int J Med Microbiol 2018; pii: S1438-4221(17)30504-0. doi: 10.1016/j.ijmm.2018.04.003. [Epub ahead of print] PubMed
  • Palankar R, Binsker U, Haracska B, Wesche J, Greinacher A, Hammerschmidt S. Interaction between the Staphylococcus aureus extracellular adherence protein Eap and its subdomains with platelets. Int J Med Microbiol 2018; pii: S1438-4221(18)30032-8. doi: 10.1016/j.ijmm.2018.04.004. [Epub ahead of print] PubMed
  • Binsker U, Palankar R, Wesche J, Kohler TP, Prucha J, Burchhardt G, Rohde M, Schmidt F, Bröker BM, Mamat U, Pané-Farré J, Graf A, Ebner P, Greinacher A, Hammerschmidt S. Secreted Immunomodulatory Proteins of Staphylococcus aureus Activate Platelets and Induce Platelet Aggregation. Thromb Haemost 2018; 118(4):745-757. PubMed
  • Graf A, Lewis R J, Fuchs S, Pagels M, Engelmann S, Riedel K, Pané-Farré J. The hidden lipoproteome of Staphylococcus aureus. Int J Med Microbiol 2018; pii: S1438-4221(17)30493-9. doi:      10.1016/j.ijmm.2018.01.008. [Epub ahead of print] PubMed
  • Leonard A, Gierok P, Methling K, Gómez-Mejia A, Hammerschmidt S, Lalk M. Metabolic inventory of Streptococcus pneumoniae growing in a chemical defined environment. Int J Med Microbiol 2018; pii: S1438-4221(17)30487-3. doi: 10.1016/j.ijmm.2018.01.001. [Epub ahead of print] PubMed
  • Hoyer J, Bartel J, Gómez-Mejia A, Rohde M, Hirschfeld C, Heß N, Sura T, Maaß S, Hammerschmidt S, Becher D. Proteomic response of Streptococcus pneumoniae to iron limitation. Int J Med Microbiol 2018; pii: S1438-4221(17)30471-X. doi: 10.1016/j.ijmm.2018.02.001. [Epub ahead of print] PubMed
  • Nguyen MT, Luqman A, Bitschar K, Hertlein T, Dick J, Ohlsen K, Bröker BM, Schittek B, Götz F. Staphylococcal (phospho)lipases promote biofilm formation and host cell invasion. Int J Med Microbiol 2018; pii: S1438-4221(17)30312-0. doi: 10.1016/j.ijmm.2017.11.013. [Epub ahead of print] PubMed
  • Schmoeckel K, Mrochen DM, Hühn J, Pötschke C, Bröker BM. Polymicrobial sepsis and non-specific immunization induce adaptive immunosuppression to a similar degree. PLoS One 2018; 13(2):e0192197. PMC
  • Gámez G, Castro A, Gómez-Mejia A, Gallego M, Bedoya A, Camargo M, Hammerschmidt S. The variome of pneumococcal virulence factors and regulators. BMC Genomics 2018; 19:10. PMC
  • König S, Romoth L, Stanke M. Comparative Genome Annotation. In: Comparative Genomics: Methods and Protocols, Methods in Molecular Biology (eds. Setubal JC et al.). Springer 2018, vol. 1704, ISBN: 978-1-4939-7461-0.
  • Gabarrini G, Palma Medina LM, Stobernack T, Prins RC, du Teil Espina M, Kuipers J, Chlebowicz MA, Rossen JWA, van Winkelhoff AJ, van Dijl JM. There's no place like OM: vesicular sorting and secretion of the peptidylarginine deiminase of Porphyromonas gingivalis. Virulence 2018; 9(1):456-464. PubMed
  • Voß F, Kohler TP, Meyer T, Abdullah MR, van Opzeeland FJ, Saleh M, Michalik S, van Selm S, Schmidt F, De Jonge MI, Hammerschmidt S. Intranasal vaccination with lipoproteins confers protection against pneumococcal colonisation. Frontiers in immunology 9:2405. doi: 10.3389/fimmu.2018.02405. eCollection 2018. PMID: 30405609 https://doi.org/10.3389/fimmu.2018.02405

2017

  • Schultz D, Schlüter R, Gerth U, Lalk M. Metabolic Perturbations in a Bacillus subtilis clpP Mutant during Glucose Starvation. Metabolites 2017, 7, 63; doi:10.3390/metabo7040063 PubMed
  • Gómez-Mejia A, Gámez G, Hammerschmidt S. Streptococcus pneumoniae Two-Component Regulatory Systems: The interplay of the pneumococcus with its environment. Int J Med Microbiol 2017; pii: S1438-4221(17)30382-X. doi: 10.1016/j.ijmm.2017.11.012. [Epub ahead of print] PubMed
  • Heß N, Waldow F, Kohler TP, Rohde M, Kreikemeyer B, Gomez Mejia A, Hain T, Schwudke D, Vollmer W, Hammerschmidt S, Gisch N. Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae. Nat Commun 2017; 8(1):2093. PubMed
  • Mrochen DM, Grumann D, Schulz D, Gumz J, Trübe P, Pritchett-Corning K, Johnson S, Nicklas W, Kirsch P, Martelet K, van den Brandt J, Berg S, Bröker BM, Wiles S, Holtfreter S. Global spread of mouse-adapted Staphylococcus aureus lineages CC1, CC15, and CC88 among mouse breeding facilities. Int J Med Microbiol 2017; pii: S1438-4221(17)30360-0. doi: 10.1016/j.ijmm.2017.11.006. [Epub ahead of print] PubMed
  • Mrochen DM, Schulz D, Fischer S, Jeske K, El Gohary H, Reil D, Imholt C, Trübe P, Suchomel J, Tricaud E, Jacob J, Heroldova M, Bröker BM, Strommenger B, Walther B, Ulrich R, Holtfreter S. Wild rodents and shrews are natural hosts of Staphylococcus aureus. Int J Med MIcrobiol 2017; pii: S1438-4221(17)30386-7. doi: 10.1016/j.ijmm.2017.09.014. [Epub ahead of print] PubMed
  • Teufelberger AR, Nordengrün M, Braun H, Maes T, De Grove K, Holtappels G, O'Brien C, Provoost S, Hammad H, Gonçalves A, Beyaert R, Declercq W, Vandenabeele P, Krysko DV, Bröker BM, Bachert C, Krysko O. The IL-33/ST2 axis is crucial in type 2 airway responses induced by the Staphylococcus aureus protease SplD. J Allergy Clin Immunol 2017; pii: S0091-6749(17)30793-5. doi: 10.1016/j.jaci.2017.05.004. [Epub ahead of print] PubMed
  • Schulz D, Grumann D, Trübe P, Pritchett-Corning K, Johnson S, Reppschläger K, Gumz J, Sundaramoorthy N, Michalik S, Berg S, van den Brandt J, Fister R, Monecke S, Uy B, Schmidt F, Bröker BM, Wiles S, Holtfreter S. Laboratory mice are frequently colonized with Staphylococcus aureus and mount a systemic immune response - Note of caution for in vivo infection experiments. Front Cell Infect Microbiol 2017; 7:152. PubMed
  • Stentzel S, Teufelberger A, Nordengrün M, Kolata J, Schmidt F, Crombruggen K, Michalik S, Kumpfmüller J, Tischer S, Schweder T, Hecker M, Engelmann S, Völker U, Krysko O, Bachert C, Bröker BM. Staphylococcal serine protease–like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus. J Allergy Clin Immunol 2017; 139(2):492-500.e8. PubMed
  • Stentzel S, Hagl B, Abel F, Kahl BC, Rack-Hoch A, Bröker BM, Renner ED. Reduced immunoglobulin (Ig) G response to Staphylococcus aureus in STAT3 hyper-IgE syndrome. Clin Infect Dis 2017; 64:1279-1282. PubMed
  • Binsker U, Kohler TP, Krauel K, Kohler S, Habermeyer J, Schwertz H, Hammerschmidt S. Serotype 3 pneumococci sequester platelet-derived human thrombospondin-1 via the adhesin and immune evasion protein Hic. J Biol Chem 2017; 292(14):5770-83. PubMed
  • Kanwal S, Jensch I, Palm GJ, Brönstrup M, Rohde M, Kohler TP, Somplatzki D, Tegge W, Jenkinson HF, Hammerschmidt S. Mapping the recognition domains of pneumococcal fibronectin-binding proteins PavA and PavB demonstrates a common pattern of molecular interactions with fibronectin type III repeats. Mol Microbiol 2017; 105(6):839-859. PubMed
  • Mekonnen SA, Palma Medina LM, Glasner C, Tsompanidou E, de Jong A, Grasso S, Schaffer M, Mäder U, Larsen AR, Gumpert H, Westh H, Völker U, Otto A, Becher D, van Dijl JM. Signatures of cytoplasmic proteins in the exoproteome distinguish community- and hospital-associated methicillin-resistant Staphylococcus aureus USA300 lineages. Virulence 2017; 8(6):891-907. PubMed
  • Michalik S, Depke M, Murr A, Gesell Salazar M, Kusebauch U, Sun Z, Meyer TC, Surmann K, Pförtner H, Hildebrandt P, Weiss S, Palma Medina LM, Gutjahr M, Hammer E, Becher D, Pribyl T, Hammerschmidt S, Deutsch EW, Bader SL, Hecker M, Moritz RL, Mäder U, Völker U, Schmidt F. A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions. Sci Reports 2017; 7:9718. Nature
  • Schmidt F, Meyer T, Sundaramoorthy N, Michalik S, Surmann K, Depke M, Dhople V, Gesell Salazar M, Holtappels G, Zhang N, Bröker BM, Bachert C, Völker U. Characterization of human and Staphylococcus aureus proteins in respiratory mucosa by in vivo- and immunoproteomics. J Proteomics 2017; 155:31-39. PubMed

2016

  • Gutiérrez-Fernández J, Saleh M, Alcorlo M, Gómez-Mejía A, Pantoja-Uceda D, Treviño MA, Voß F, Abdullah MR, Galán-Bartual S, Seinen J, Sánchez-Murcia PA, Gago F, Bruix M, Hammerschmidt S, Hermoso JA. Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis. Sci Rep. 2016; 6:38094. PubMed
  • Kohler S, Voß F, Gómez Mejia A, Brown JS, Hammerschmidt S. Pneumococcal lipoproteins involved in bacterial fitness, virulence, and immune evasion. FEBS Lett 2016; 590(21):3820-3839. PubMed
  • Gierok P, Harms M, Methling K, Hochgräfe F, Lalk M. Staphylococcus aureus Infection Reduces Nutrition Uptake and Nucleotide Biosynthesis in a Human Airway Epithelial Cell Line. Metabolites 2016; 6(4):41. PubMed
  • Vu CH, Kolata J, Stentzel S, Beyer A, Gesell Salazar M, Steil L, Pané-Farré J, Rühmling V, Engelmann S, Götz F, van Dijl JM, Hecker M, Mäder U, Schmidt F, Völker U, Bröker BM. Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects. Proteomics 2016; 16(20):2667-2677. PubMed
  • Ribes S, Abdullah MR, Saleh M, Hanisch UK, Nau R, Hammerschmidt S. Thioredoxins and Methionine Sulfoxide Reductases in the Pathophysiology of Pneumococcal Meningitis. J Infect Dis 2016; 214(6):953-961. PubMed
  • Stentzel S, Gläser R, Bröker BM. Elucidating the anti-Staphylococcus aureus antibody response by immunoproteomics. Proteomics Clin. Appl. 2016; 10:1011–1019. PubMed
  • König S, Romoth LW, Gerischer L, Stanke M. Simultaneous gene finding in multiple genomes. Bioinformatics 2016; 32(22):3388-3395. Oxford Journals
  • Zühlke D, Dörries K, Bernhardt J, Maaß S, Muntel J, Liebscher V, Pané-Farré J, Riedel K, Lalk M, Völker U, Engelmann S, Becher D, Fuchs S, Hecker M. Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. Sci Rep 2016; 6:28172. PubMed
  • Mäder U, Nicolas P, Depke M, Pané-Farré J, Debarbouille M, van der Kooi-Pol MM, Guérin C, Dérozier S, Hiron A, Jarmer H, Leduc A, Michalik S, Reilman E, Schaffer M, Schmidt F, Bessières P, Noirot P, Hecker M, Msadek T, Völker U, van Dijl JM. Staphylococcus aureus transcriptome architecture: From laboratory to infection-mimicking conditions. PLoS Genet 2016; 12(4):e1005962. PubMed
  • Iovino F, Seinen J, Henriques-Normark B, van Dijl JM. How Does Streptococcus pneumoniae Invade the Brain? Trends Microbiol 2016; 24(4):307-315. PubMed
  • Gierok P, Kohler C, Steinmetz I, Lalk M. Burkholderia pseudomallei colony morphotypes show a synchronized metabolic pattern after acute infection. PLoS Negl Trop Dis 2016; 10(3):e0004483. PubMed
  • Bröker BM, Mrochen D, Péton V. The T Cell Response to Staphylococcus aureus. Pathogens 2016; 5(3). doi:10.3390/pathogens5010031. PubMed
  • Holtfreter S, Kolata J, Stentzel S, Bauerfeind S, Schmidt F, Sundaramoorthy N, Bröker BM. Omics Approaches for the Study of Adaptive Immunity to Staphylococcus aureus and the Selection of Vaccine Candidates. Proteomes 2016; 4(11). doi:10.3390/proteomes4010011. MDPI
  • Liang C, Schaack D, Srivastava M, Gupta SK, Sarukhanyan E, Giese A, Pagels M, Romanov N, Pané-Farré J, Fuchs S, Dandekar T. A Staphylococcus aureus proteome overview: Shared and specific proteins and protein complexes from representative strains of all three clades. Proteomes 2016; 4(1):8. PubMed

2015

  • Binsker U, Kohler TP, Krauel K, Kohler S, Schwertz H, Hammerschmidt S. Pneumococcal adhesins PavB and PspC are important for the interplay with human thrombospondin-1. J Biol Chem 2015; 290(23):14542-55. PubMed
  • Hilleringmann M, Kohler S, Gàmez G, Hammerschmidt S. Pneumococcal pili and adhesins. In: Streptococcus pneumoniae: Molecular mechanisms of host-pathogen interactions (eds. Jeremy Brown, Sven Hammerschmidt, Carlos Orihuela). p. 309-346. ELSEVIER 2015, Amsterdam, NL. ISBN: 978-0-12-410530-0. Elsevier
  • Bergmann S, Agarwal V, Hammerschmidt S. Exploitation of host signal transduction pathways induced by Streptococcus pneumoniae. In: Streptococcus pneumoniae: Molecular mechanisms of host-pathogen interactions (eds. Jeremy Brown, Sven Hammerschmidt, Carlos Orihuela). p. 347-362. ELSEVIER 2015, Amsterdam, NL. ISBN: 978-0-12-410530-0. Elsevier
  • Muschter S, Berthold T, Bhardwaj G, Hammer E, Dhople VM, Wesche J, Reil A, Bux J, Bakchoul T, Steil L, Greinacher A, Völker U. Mass spectrometric phosphoproteome analysis of small-sized samples of human neutrophils. Clinica Chimica Acta 2015; 451:199–207. PubMed

2014

  • Schulz C, Gierok P, Petruschka L, Lalk M, Mäder U, Hammerschmidt S.Regulation of the arginine deiminase system by ArgR2 interferes with arginine metabolism and fitness of Streptococcus pneumoniae. MBio 2014; 5(6). pii: e01858-14. doi: 10.1128/mBio.01858-14. PubMed
  • Dörries K, Schlüter R, Lalk M. Impact of antibiotics with various target sites on the metabolome of Staphylococcus aureus. Antimicrob Agents Chemother 2014; 58(12):7151-63. PubMed
  • Müller M, Reiß S, Schlüter R, Mäder U, Beyer A, Reiß W, Marles-Wright J, Lewis RJ, Pförtner H, Völker U, Riedel K, Hecker M, Engelmann S, Pané-Farré J. Deletion of membrane-associated Asp23 leads to upregulation of cell wall stress genes in Staphylococcus aureus. Mol Microbiol 2014; 93(6):1259-68. doi: 10.1111/mmi.12733. PubMed
  • Abdullah MR, Gutiérrez-Fernández J, Pribyl T, Gisch N, Saleh M, Rohde M, Petruschka L, Burchhardt G, Schwudke D, Hermoso JA, Hammerschmidt S. Structure of the pneumococcal l,d-carboxypeptidase DacB and pathophysiological effects of disabled cell wall hydrolases DacA and DacB. Mol Microbiol 2014; 93(6):1183-206. doi: 10.1111/mmi.12729. PubMed