Dr. Leif Steil
Gruppenleiter
Interfakultäres Institut für Genetik und Funktionelle Genomforschung
Felix-Hausdorff-Straße 8
D-17475 Greifswald
Büro: Raum 2.61
Telefon +49 3834 420 5810
Telefax +49 3834 420 5890
Publikationen
2024
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Abdurrahman G, Pospich R, Steil L, Gesell Salazar M, Izquierdo González JJ, Normann N, Mrochen D, Scharf C, Völker U, Werfel T, Bröker BM, Roesner LM, Gómez-Gascón L (2024) The extracellular serine protease from Staphylococcus epidermidis elicits a type 2-biased immune response in atopic dermatitis patients. Front Immunol. 15:1352704. ArtikelPubmedRelated |
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Alcorlo M, Abdullah MR, Steil L, Sotomayor F, López-de Oro L, de Castro S, Velázquez S, Kohler TP, Jiménez E, Medina A, Usón I, Keller LE, Bradshaw JL, McDaniel LS, Camarasa M, Völker U, Hammerschmidt S, Hermoso JA (2024) Molecular and structural basis of oligopeptide recognition by the Ami transporter system in pneumococci. PLoS Pathog. 20:e1011883. ArtikelPubmedRelated |
| Reder A, Hentschker C, Steil L, Gesell Salazar M, Hammer E, Dhople VM, Sura T, Lissner U, Wolfgramm H, Dittmar D, Harms M, Surmann K, Völker U, Michalik S (2024) MassSpecPreppy-An end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications. Proteomics. 24:e2300294. ArtikelPubmedRelated |
2023
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Öktem A, Núñez-Nepomuceno D, Ferrero-Bordera B, Walgraeve J, Seefried M, Gesell Salazar M, Steil L, Michalik S, Maaß S, Becher D, Mäder U, Völker U, van Dijl JM (2023) Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of Bacillus subtilis. Microbiol Spectr. 11:e0177823. ArtikelPubmedRelated |
2022
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Dasari P, Nordengrün M, Vilhena C, Steil L, Abdurrahman G, Surmann K, Dhople V, Lahrberg J, Bachert C, Skerka C, Völker U, Bröker BM, Zipfel PF (2022) The Protease SplB of Staphylococcus aureus Targets Host Complement Components and Inhibits Complement-Mediated Bacterial Opsonophagocytosis J Bacteriol. 204:e0018421. ArtikelPubmedRelated |
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Greinacher A, Schönborn L, Siegerist F, Steil L, Palankar R, Handtke S, Reder A, Thiele T, Aurich K, Methling K, Lalk M, Völker U, Endlich N (2022) Pathogenesis of vaccine-induced immune thrombotic thrombocytopenia (VITT). Semin. Hematol. 59:97-107. ArtikelPubmedRelated |
| Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, Völker U (2022) Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines. Haematologica. ArtikelPubmedRelated |
2021
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Dasari P, Nordengrün M, Steil L, Dhople V, Lahrberg J, Bachert C, Skerka C, Völker U, Bröker BM, Zipfel PF (2021) The protease SplB of Staphylococcus aureus targets host complement components and inhibits complement-mediated bacterial opsonophagocytosis. J Bacteriol. ArtikelPubmedRelated |
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Greinacher A, Selleng K, Palankar R, Wesche J, Handtke S, Wolff M, Aurich K, Lalk M, Methling K, Volker U, Hentschker C, Michalik S, Steil L, Reder A, Schönborn L, Beer M, Franzke K, Büttner A, Fehse B, Stavrou EX, Rangaswamy C, Mailer RK, Englert H, Frye M, Thiele T, Kochanek S, Krutzke L, Siegerist F, Endlich N, Warkentin TE, Renné T (2021) Insights in ChAdOx1 nCov-19 Vaccine-induced Immune Thrombotic Thrombocytopenia (VITT). Blood. ArtikelPubmedRelated |
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Prajapati B, Bernal-Cabas M, López-Álvarez M, Schaffer M, Bartel J, Rath H, Steil L, Becher D, Völker U, Mäder U, van Dijl JM (2021) Double trouble: Bacillus depends on a functional Tat machinery to avoid severe oxidative stress and starvation upon entry into a NaCl-depleted environment. Biochim Biophys Acta Mol Cell Res. 1868:118914. ArtikelPubmedRelated |
2020
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Normann N, Tietz G, Kühn A, Fuchs C, Balau V, Schulz K, Kolata J, Schuerholz T, Petersmann A, Stentzel S, Steil L, Kuhn SO, Meissner K, Völker U, Nauck M, Steinmetz I, Raafat D, Gründling M, Bröker BM (2020) Pathogen-specific antibody profiles in patients with severe systemic infections. Eur Cell Mater. 39:171-182. ArtikelPubmedRelated |
| Rath H, Sappa PK, Hoffmann T, Salazar MG, Reder A, Steil L, Hecker M, Bremer E, Mäder U, Völker U (2020) Impact of high salinity and the compatible solute glycine betaine on gene expression of Bacillus subtilis. Environ Microbiol. ArtikelPubmedRelated |
2019
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Bartolini M, Cogliati S, Vileta D, Bauman C, Rateni L, Leñini C, Argañaraz F, Francisco M, Villalba JM, Steil L, Völker U, Grau R (2019) Regulation of Biofilm Aging and Dispersal in Bacillus subtilis by the Alternative Sigma Factor SigB. J Bacteriol. 201:1-14. ArtikelPubmedRelated |
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Handtke S, Steil L, Palankar R, Conrad J, Cauhan S, Kraus L, Ferrara M, Dhople V, Wesche J, Völker U, Greinacher A, Thiele T (2019) Role of Platelet Size Revisited-Function and Protein Composition of Large and Small Platelets. Thromb Haemost. 119:407-420. ArtikelPubmedRelated |
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Mugahid DA, Sengul TG, You X, Wang Y, Steil L, Bergmann N, Radke MH, Ofenbauer A, Gesell-Salazar M, Balogh A, Kempa S, Tursun B, Robbins CT, Völker U, Chen W, Nelson L, Gotthardt M (2019) Proteomic and Transcriptomic Changes in Hibernating Grizzly Bears Reveal Metabolic and Signaling Pathways that Protect against Muscle Atrophy. Sci Rep. 9:19976. ArtikelPubmedRelated |
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Scharf C, Eymann C, Emicke P, Bernhardt J, Wilhelm M, Görries F, Winter J, von Woedtke T, Darm K, Daeschlein G, Steil L, Hosemann W, Beule A (2019) Improved Wound Healing of Airway Epithelial Cells Is Mediated by Cold Atmospheric Plasma: A Time Course-Related Proteome Analysis. Oxid Med Cell Longev. 2019:7071536. ArtikelPubmedRelated |
2017
| Bhardwaj G, Dörr M, Sappa PK, Ameling S, Dhople V, Steil L, Klingel K, Empen K, Beug D, Völker U, Felix SB, Hammer E (2017) Endomyocardial proteomic signature corresponding to the response of patients with dilated cardiomyopathy to immunoadsorption therapy. J Proteomics. 150:121-129. ArtikelPubmedRelated |
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Hoffmann T, Bleisteiner M, Sappa PK, Steil L, Mäder U, Völker U, Bremer E (2017) Synthesis of the compatible solute proline by : point mutations rendering the osmotically controlled proHJ promoter hyperactive. Environ Microbiol. 19:3700-3720. ArtikelPubmedRelated |
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Reuß DR, Altenbuchner J, Mäder U, Rath H, Ischebeck T, Sappa PK, Thürmer A, Guérin C, Nicolas P, Steil L, Zhu B, Feussner I, Klumpp S, Daniel R, Commichau FM, Völker U, Stülke J (2017) Large-scale reduction of the genome: consequences for the transcriptional network, resource allocation, and metabolism. Genome Res. 27:289-299. ArtikelPubmedRelated |
2016
| Ameling S, Bhardwaj G, Hammer E, Beug D, Steil L, Reinke Y, Weitmann K, Grube M, Trimpert C, Klingel K, Kandolf R, Hoffmann W, Nauck M, Dörr M, Empen K, Felix SB, Völker U (2016) Changes of myocardial gene expression and protein composition in patients with dilated cardiomyopathy after immunoadsorption with subsequent immunoglobulin substitution. Basic Res Cardiol. 111:53. ArtikelPubmedRelated |
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Mangner N, Bowen TS, Werner S, Fischer T, Kullnick Y, Oberbach A, Linke A, Steil L, Schuler G, Adams V (2016) Exercise Training Prevents Diaphragm Contractile Dysfunction in Heart Failure. Med Sci Sports Exerc. 48:2118-2124. ArtikelPubmedRelated |
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Thiele T, Braune J, Dhople V, Hammer E, Scharf C, Greinacher A, Völker U, Steil L (2016) Proteomic profile of platelets during reconstitution of platelet counts after apheresis. Proteomics Clin Appl. 10:831-8. ArtikelPubmedRelated |
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Vu CH, Kolata J, Stentzel S, Beyer A, Gesell Salazar M, Steil L, Pané-Farré J, Rühmling V, Engelmann S, Götz F, van Dijl JM, Hecker M, Mäder U, Schmidt F, Völker U, Bröker BM (2016) Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects. Proteomics. 16:2667-2677. ArtikelPubmedRelated |
2015
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Bowen TS, Mangner N, Werner S, Glaser S, Kullnick Y, Schrepper A, Doenst T, Oberbach A, Linke A, Steil L, Schuler G, Adams V (2015) Diaphragm muscle weakness in mice is early-onset post-myocardial infarction and associated with elevated protein oxidation. J Appl Physiol (1985). 118:11-9. ArtikelPubmedRelated |
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Haring R, Schurmann C, Homuth G, Steil L, Völker U, Völzke H, Keevil BG, Nauck M, Wallaschofski H (2015) Associations between Serum Sex Hormone Concentrations and Whole Blood Gene Expression Profiles in the General Population. PLoS One. 10:e0127466. ArtikelPubmedRelated |
| Homuth G, Wahl S, Müller C, Schurmann C, Mäder U, Blankenberg S, Carstensen M, Dörr M, Endlich K, Englbrecht C, Felix SB, Gieger C, Grallert H, Herder C, Illig T, Kruppa J, Marzi CS, Mayerle J, Meitinger T, Metspalu A, Nauck M, Peters A, Rathmann W, Reinmaa E, Rettig R, Roden M, Schillert A, Schramm K, Steil L, Strauch K, Teumer A, Völzke H, Wallaschofski H, Wild PS, Ziegler A, Völker U, Prokisch H, Zeller T (2015) Extensive alterations of the whole-blood transcriptome are associated with body mass index: results of an mRNA profiling study involving two large population-based cohorts. BMC Med Genomics. 8:65. ArtikelPubmedRelated |
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Kolata JB, Kühbandner I, Link C, Normann N, Vu CH, Steil L, Weidenmaier C, Bröker BM (2015) The Fall of a Dogma? Unexpected High T-Cell Memory Response to Staphylococcus aureus in Humans. J Infect Dis. 212:830-8. ArtikelPubmedRelated |
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Muschter S, Berthold T, Bhardwaj G, Hammer E, Dhople VM, Wesche J, Reil A, Bux J, Bakchoul T, Steil L, Greinacher A, Völker U (2015) Mass spectrometric phosphoproteome analysis of small-sized samples of human neutrophils. Clin Chim Acta. 451:199-207. ArtikelPubmedRelated |
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Peters MJ, Joehanes R, Pilling LC, Schurmann C, Conneely KN, Powell J, Reinmaa E, Sutphin GL, Zhernakova A, Schramm K, Wilson YA, Kobes S, Tukiainen T, NABEC/UKBEC Consortium , Ramos YF, Göring HHH, Fornage M, Liu Y, Gharib SA, Stranger BE, De Jager PL, Aviv A, Levy D, Murabito JM, Munson PJ, Huan T, Hofman A, Uitterlinden AG, Rivadeneira F, van Rooij J, Stolk L, Broer L, Verbiest MMPJ, Jhamai M, Arp P, Metspalu A, Tserel L, Milani L, Samani NJ, Peterson P, Kasela S, Codd V, Peters A, Ward-Caviness CK, Herder C, Waldenberger M, Roden M, Singmann P, Zeilinger S, Illig T, Homuth G, Grabe H, Völzke H, Steil L, Kocher T, Murray A, Melzer D, Yaghootkar H, Bandinelli S, Moses EK, Kent JW, Curran JE, Johnson MP, Williams-Blangero S, Westra H, McRae AF, Smith JA, Kardia SLR, Hovatta I, Perola M, Ripatti S, Salomaa V, Henders AK, Martin NG, Smith AK, Mehta D, Binder EB, Nylocks KM, Kennedy EM, Klengel T, Ding J, Suchy-Dicey AM, Enquobahrie DA, Brody J, Rotter JI, Chen YI, Houwing-Duistermaat J, Kloppenburg M, Slagboom PE, Helmer Q, den Hollander W, Bean S, Raj T, Bakhshi N, Wang QP, Oyston LJ, Psaty BM, Tracy RP, Montgomery GW, Turner ST, Blangero J, Meulenbelt I, Ressler KJ, Yang J, Franke L, Kettunen J, Visscher PM, Neely GG, Korstanje R, Hanson RL, Prokisch H, Ferrucci L, Esko T, Teumer A, van Meurs JBJ, Johnson AD (2015) The transcriptional landscape of age in human peripheral blood. Nat Commun. 6:8570. ArtikelPubmedRelated |
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Stentzel S, Sundaramoorthy N, Michalik S, Nordengrün M, Schulz S, Kolata J, Kloppot P, Engelmann S, Steil L, Hecker M, Schmidt F, Völker U, Roghmann M, Bröker BM (2015) Specific serum IgG at diagnosis of Staphylococcus aureus bloodstream invasion is correlated with disease progression. J Proteomics. 128:1-7. ArtikelPubmedRelated |
| Surmann K, Simon M, Hildebrandt P, Pförtner H, Michalik S, Stentzel S, Steil L, Dhople VM, Bernhardt J, Schlüter R, Depke M, Gierok P, Lalk M, Bröker BM, Schmidt F, Völker U (2015) A proteomic perspective of the interplay of Staphylococcus aureus and human alveolar epithelial cells during infection. J Proteomics. 128:203-17. ArtikelPubmedRelated |
2014
| Depke M, Breitbach K, Dinh Hoang Dang K, Brinkmann L, Salazar MG, Dhople VM, Bast A, Steil L, Schmidt F, Steinmetz I, Völker U (2014) Bone marrow-derived macrophages from BALB/c and C57BL/6 mice fundamentally differ in their respiratory chain complex proteins, lysosomal enzymes and components of antioxidant stress systems. J Proteomics. 103:72-86. ArtikelPubmedRelated |
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Kohlstedt M, Sappa PK, Meyer H, Maaß S, Zaprasis A, Hoffmann T, Becker J, Steil L, Hecker M, van Dijl JM, Lalk M, Mäder U, Stülke J, Bremer E, Völker U, Wittmann C (2014) Adaptation of carbon core metabolism to simultaneous nutrient limitation and osmotic challenge: a multi-omics perspective. Environ Microbiol. 16:1898-917. ArtikelPubmedRelated |
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Prudent M, D'Alessandro A, Cazenave J, Devine DV, Gachet C, Greinacher A, Lion N, Schubert P, Steil L, Thiele T, Tissot J, Völker U, Zolla L (2014) Proteome changes in platelets after pathogen inactivation--an interlaboratory consensus. Transfus Med Rev. 28:72-83. ArtikelPubmedRelated |
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Stentzel S, Vu HC, Weyrich AM, Jehmlich N, Schmidt F, Salazar MG, Steil L, Völker U, Bröker BM (2014) Altered immune proteome of Staphylococcus aureus under iron-restricted growth conditions. Proteomics. 14:1857-67. ArtikelPubmedRelated |
2013
| Ameling S, Herda LR, Hammer E, Steil L, Teumer A, Trimpert C, Dörr M, Kroemer HK, Klingel K, Kandolf R, Völker U, Felix SB (2013) Myocardial gene expression profiles and cardiodepressant autoantibodies predict response of patients with dilated cardiomyopathy to immunoadsorption therapy. Eur Heart J. 34:666-75. ArtikelPubmedRelated |
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Hoffmann T, Wensing A, Brosius M, Steil L, Völker U, Bremer E (2013) Osmotic control of opuA expression in and its modulation in response to intracellular glycine betaine and proline pools. J Bacteriol. 195:510-22. ArtikelPubmedRelated |
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Jehmlich N, Dinh KHD, Gesell-Salazar M, Hammer E, Steil L, Dhople VM, Schurmann C, Holtfreter B, Kocher T, Völker U (2013) Quantitative analysis of the intra- and inter-subject variability of the whole salivary proteome. J Periodontal Res. 48:392-403. ArtikelPubmedRelated |
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Nishtala K, Phong TQ, Steil L, Sauter M, Salazar MG, Kandolf R, Felix SB, Völker U, Klingel K, Hammer E (2013) Proteomic analyses of age related changes in A.BY/SnJ mouse hearts. Proteome Sci. 11:29. ArtikelPubmedRelated |
| Pförtner H, Wagner J, Surmann K, Hildebrandt P, Ernst S, Bernhardt J, Schurmann C, Gutjahr M, Depke M, Jehmlich U, Dhople V, Hammer E, Steil L, Völker U, Schmidt F (2013) A proteomics workflow for quantitative and time-resolved analysis of adaptation reactions of internalized bacteria. Methods. 61:244-50. ArtikelPubmedRelated |
2012
| Hammer E, Phong TQ, Steil L, Gesell Salazar M, Scharf C, Kandolf R, Felix SB, Kroemer HK, Klingel K, Völker U (2012) Comparative immunoproteome analysis of the response of susceptible A.BY/SnJ and resistant C57BL/6 mice to Coxsackievirus B3-infection J Integr OMICS. 2:54-63. ArtikelPubmedRelated |
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Hussner J, Ameling S, Hammer E, Herzog S, Steil L, Schwebe M, Niessen J, Schroeder HWS, Kroemer HK, Ritter CA, Völker U, Bien S (2012) Regulation of interferon-inducible proteins by doxorubicin via interferon $\gamma$-Janus tyrosine kinase-signal transducer and activator of transcription signaling in tumor cells. Mol Pharmacol. 81:679-88. ArtikelPubmedRelated |
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Liebal UW, Sappa PK, Millat T, Steil L, Homuth G, Völker U, Wolkenhauer O (2012) Proteolysis of beta-galactosidase following SigmaB activation in . Mol Biosyst. 8:1806-14. ArtikelPubmedRelated |
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Nannapaneni P, Hertwig F, Depke M, Hecker M, Mäder U, Völker U, Steil L, van Hijum SAFT (2012) Defining the structure of the general stress regulon of using targeted microarray analysis and random forest classification. Microbiology. 158:696-707. ArtikelPubmedRelated |
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Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RAT, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P (2012) Condition-dependent transcriptome reveals high-level regulatory architecture in . Science. 335:1103-6. ArtikelPubmedRelated |
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Thiele T, Sablewski A, Iuga C, Bakchoul T, Bente A, Görg S, Völker U, Greinacher A, Steil L (2012) Profiling alterations in platelets induced by Amotosalen/UVA pathogen reduction and gamma irradiation--a LC-ESI-MS/MS-based proteomics approach. Blood Transfus. 10 Suppl 2:s63-70. ArtikelPubmedRelated |
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Thiele T, Iuga C, Janetzky S, Schwertz H, Gesell Salazar M, Fürll B, Völker U, Greinacher A, Steil L (2012) Early storage lesions in apheresis platelets are induced by the activation of the integrin $\alpha$IIb$\beta$₃ and focal adhesion signaling pathways. J Proteomics. 76 Spec No:297-315. ArtikelPubmedRelated |
2011
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Gebhard S, Steil L, Peters B, Gesell-Salazar M, Hammer E, Kuttler B, Clemetson KJ, Scharf C, Peters J, Völker U, Rettig R, Greinacher A (2011) Angiotensin II-dependent hypertension causes reversible changes in the platelet proteome. J Hypertens. 29:2126-37. ArtikelPubmedRelated |
| Hammer E, Goritzka M, Ameling S, Darm K, Steil L, Klingel K, Trimpert C, Herda LR, Dörr M, Kroemer HK, Kandolf R, Staudt A, Felix SB, Völker U (2011) Characterization of the human myocardial proteome in inflammatory dilated cardiomyopathy by label-free quantitative shotgun proteomics of heart biopsies. J Proteome Res. 10:2161-71. ArtikelPubmedRelated |
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Holtfreter S, Jursa-Kulesza J, Masiuk H, Verkaik NJ, de Vogel C, Kolata J, Nowosiad M, Steil L, van Wamel W, van Belkum A, Völker U, Giedrys-Kalemba S, Bröker BM (2011) Antibody responses in furunculosis patients vaccinated with autologous formalin-killed Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 30:707-17. ArtikelPubmedRelated |
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Kolata J, Bode LGM, Holtfreter S, Steil L, Kusch H, Holtfreter B, Albrecht D, Hecker M, Engelmann S, van Belkum A, Völker U, Bröker BM (2011) Distinctive patterns in the human antibody response to Staphylococcus aureus bacteremia in carriers and non-carriers. Proteomics. 11:3914-27. ArtikelPubmedRelated |
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Nishtala K, Phong TQ, Steil L, Sauter M, Salazar MG, Kandolf R, Kroemer HK, Felix SB, Völker U, Klingel K, Hammer E (2011) Virus-induced dilated cardiomyopathy is characterized by increased levels of fibrotic extracellular matrix proteins and reduced amounts of energy-producing enzymes. Proteomics. 11:4310-20. ArtikelPubmedRelated |
2010
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Hahne H, Mäder U, Otto A, Bonn F, Steil L, Bremer E, Hecker M, Becher D (2010) A comprehensive proteomics and transcriptomics analysis of salt stress adaptation. J Bacteriol. 192:870-82. ArtikelPubmedRelated |
| Hammer E, Bien S, Salazar MG, Steil L, Scharf C, Hildebrandt P, Schroeder HWS, Kroemer HK, Völker U, Ritter CA (2010) Proteomic analysis of doxorubicin-induced changes in the proteome of HepG2cells combining 2-D DIGE and LC-MS/MS approaches. Proteomics. 10:99-114. ArtikelPubmedRelated |
| Hammer E, Phong TQ, Steil L, Klingel K, Salazar MG, Bernhardt J, Kandolf R, Kroemer HK, Felix SB, Völker U (2010) Viral myocarditis induced by Coxsackievirus B3 in A.BY/SnJ mice: analysis of changes in the myocardial proteome. Proteomics. 10:1802-18. ArtikelPubmedRelated |
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Thiele T, Steil L, Völker U, Greinacher A (2010) Transfusion medicine and proteomics. Alliance or coexistence? Blood Transfus. 8 Suppl 3:s16-25. ArtikelPubmedRelated |